Bar-Ilan University (BIU)- Research Group

Prof. Shulamit Michaeli

Affiliation
The Mina & Everard Goodman Faculty of Life Sciences
Institute of Nanotechnology and Advanced Materials
Bar-Ilan University

Contact Information
Prof. Shulamit Michaeli
Building: 206, 3rd floor
Room: C- 369, 371
Office phone: 972-3-5318068
Lab phone: 972-3-5317522
Office fax: 972-3-7384058
Email: Shulamit.Michaeli@biu.ac.il
Home page: http://www.biu.ac.il/faculty/michaes

Mailing adress
The Mina & Everard Goodman Faculty of Life Sciences
Institute of Nanotechnology and Advanced Materials
Bar-Ilan University
Ramat-Gan 52900, Israel

Research Interest

    Our model systems are the trypanosomes, parasitic protozoa that are the causative agent of devastating parasitic diseases such as sleeping sickness, leishmaniasis and Chagas’ disease, which affect millions of people worldwide. In my laboratory, we are investigating processes which are unique to the parasite and not found in the mammalian host; our hope is to find unique targets for therapy.
  • We focus on the structure and function of RNA molecules that participate in RNA processing trans-splicing that is unique to these parasites. We are interested in the structure and function of novel anti-sense non-coding RNAs and the mechanism of a novel RNAi silencing event discovered in our laboratory, snoRNAi, which silences nucleolar RNAs. Recently, we discovered a novel stress-induced mechanism that silences the production of mRNA by abolishing trans-splicing that leads to apoptosis. We term this process SLS, and plan to identify chemical compounds that elicit this death pathway as potential chemotherapy.
  • We study the mechanism of protein sorting across the ER, especially the role of the signal recognition particles (SR), since we found that the trypanosome particle carries two RNA molecules unlike all its homologues in other eukaryotes. In addition, we study unique RNA quality control mechanisms that regulate the level of non-coding RNAs.
  • My laboratory is also investigating nuclear RNA silencing in human cells and exploring the use of nanotechnology for gene silencing.
  • We use a variety of methodologies from generating of transgenic parasites, knock-out, RNAi silencing, biochemical fractionation of RNA-protein complexes, microarray analysis of the transcriptome, RNA-seq of small RNome, and live cell imaging of RNA, proteins and more.

Selected Publications

Michaeli S, Doniger T, Gupta SK, Wurtzel O, Romano M, Visnovezky D, Sorek R, Unger R, Ullu E. RNA-seq analysis of small RNPs in Trypanosoma brucei reveals a rich repertoire of non-coding RNAs. (2012) Nucleic Acids Res. 40(3):1282-1298.

Michaeli S. Trans-splicing in trypanosomes: machinery and its impact on the parasite transcriptome. (2011) Future Microbiol. 6(4):459-474.

Wachtel C, Michaeli S. Functional analysis of noncoding RNAs in trypanosomes: RNA walk, a novel approach to study RNA-RNA interactions between small RNA and its target. (2011) Methods Mol Biol. 718:245-257.

Goldshmidt H, Michaeli S. Induction of ER stress response leading to programmed cell death in Trypanosoma brucei. (2011) Methods Enzymol. 489:189-205.

Gupta SK, Hury A, Ziporen Y, Shi H, Ullu E, Michaeli S. Small nucleolar RNA interference in Trypanosoma brucei: mechanism and utilization for elucidating the function of snoRNAs. (2010) Nucleic Acids Res. 38(20):7236-7247.

Tkacz ID, Gupta SK, Volkov V, Romano M, Haham T, Tulinski P, Lebenthal I, Michaeli S. Analysis of spliceosomal proteins in Trypanosomatids reveals novel functions in mRNA processing. (2010) J Biol Chem. 285(36): 27982-27999.

Shaked H, Wachtel C, Tulinski P, Yahia NH, Barda O, Darzynkiewicz E, Nilsen TW, Michaeli S. Establishment of an in vitro trans-splicing system in Trypanosoma brucei that requires endogenous spliced leader RNA. (2010) Nucleic Acids Res. 38(10): e114.

Goldshmidt H, Matas D, Kabi A, Carmi S, Hope R, Michaeli S. Persistent ER stress induces the spliced leader RNA silencing pathway (SLS), leading to programmed cell death in Trypanosoma brucei. (2010) PLoS Pathog. 6(1): e1000731.

Lustig Y, Wachtel C, Safro M, Liu L, Michaeli S. 'RNA walk' a novel approach to study RNA-RNA interactions between a small RNA and its target. (2010)Nucleic Acids Res 38(1): e5.

Michaeli S. Trans-splicing is a regulated and is master regulator of trypanosome gene expression (2010) Febs Journal, 277, 20-20.

Hury A, Goldshmidt H, Tkacz ID, Michaeli S. Trypanosome spliced-leader-associated RNA (SLA1) localization and implications for spliced-leader RNA biogenesis. (2009) Eukaryot Cell. 8(1):56-68.

Goldshmidt H, Sheiner L, Bütikofer P, Roditi I, Uliel S, Günzel M, Engstler M, Michaeli S. (2008) Role of protein translocation pathways across the endoplasmic reticulum in Trypanosoma brucei. J Biol Chem. 2008 283(46):32085-32098.

Tkacz ID, Cohen S, Salmon-Divon M, Michaeli S. Identification of the heptameric Lsm complex that binds U6 snRNA in Trypanosoma brucei.(2008) Mol Biochem Parasitol. 160(1):22-31.

Barth S, Shalem B, Hury A, Tkacz ID, Liang XH, Uliel S, Myslyuk I, Doniger T, Salmon-Divon M, Unger R, Michaeli S. Elucidating the role of C/D snoRNA in rRNA processing and modification in Trypanosoma brucei. (2008) Eukaryot Cell. 7(1):86-101.

Lustig Y, Vagima Y, Goldshmidt H, Erlanger A, Ozeri V, Vince J, McConville MJ, Dwyer DM, Landfear SM, Michaeli S. Down-regulation of the trypanosomatid signal recognition particle affects the biogenesis of polytopic membrane proteins but not of signal peptide-containing proteins. (2007) Eukaryot Cell. 6(10):1865-1875.

Lustig Y, Sheiner L, Vagima Y, Goldshmidt H, Das A, Bellofatto V, Michaeli S. Spliced-leader RNA silencing: a novel stress-induced mechanism in Trypanosoma brucei. (2007) EMBO Rep. 8(4):408-13.

Liang XH, Hury A, Hoze E, Uliel S, Myslyuk I, Apatoff A, Unger R, Michaeli S. Genome-wide analysis of C/D and H/ACA-like small nucleolar RNAs in Leishmania major indicates conservation among trypanosomatids in the repertoire and in their rRNA targets. (2007) Eukaryot Cell. 6(3):361-377.

Tkacz ID, Lustig Y, Stern MZ, Biton M, Salmon-Divon M, Das A, Bellofatto V, Michaeli S. Identification of novel snRNA-specific Sm proteins that bind selectively to U2 and U4 snRNAs in Trypanosoma brucei. (2007) RNA. 13(1):30-43.

Full list of publication

Research Groups

Tel Aviv University (TAU)

Ben-Gurion University of the Negev (BGU)

The Hebrew University of Jerusalem (HUJI)

Bar-Ilan University (BIU)

  • Shulamit Michaeli

The Chaim Sheba Medical Center (SMC)